... (I & II) were used to build the ATP KPI surveys. Surveys III and IV were collected after treatment was initiated for at least 6 months. Survey IV was targeted to Field A, a field that received the earliest biocide treatments. Genera that showed at least 10% abundance were included for analysis...

...The activity of cells in immediately fixated samples was measured by determining the RNA content by RT-qPCR targeting 16S rRNA molecules for each of the specific groups cited in the previous paragraph. Figure 2 gives an overview of the methodology applied to evaluate biocide efficacy against...

...When choosing a biocide, it is well understood that microbial fouling in oil and gas stimulation causes a multitude of problems, including formation plugging, H₂S exposure, microbial induced corrosion, and well souring, all of which have deleterious effects on well production and quality...

... to the laboratory for testing. A study was completed on sludge sampled from a crude oil transmission pipeline in order to (i) observe the impact of biocide, biodispersant, and combined biocide-biodispersant treatment on sludge microbial communities and MIC, and (ii) compare molecular microbiological methods...

...Abstract The lengthy laterals of horizontal wells often pose microbiological challenges, as they provide more area to become microbially contaminated and require larger volumes of fluid and biocide for treatment. A Permian Basin oilfield has been experiencing MIC-related failures in its horizontal...

...Abstract Hydraulic fracturing operations are prone to microbial contamination due to the large volumes of water used. Therefore, biocides are often added to hydraulic fracturing fluids to control microorganisms that cause operational problems such as souring, corrosion, and decreased oil and gas...

...Abstract Hydrogen sulfide gas produced by sulfate reducing microorganisms (SRM) creates significant challenges in the petroleum industry including corrosion concerns, product devaluation, and significant health risks. Biocides and inhibitors are often employed to control these detrimental...

...Souring Minimum Cidal Concentration (MCC) Sulfide Biocide INTRODUCTION Microbial contamination in oil and gas applications results in a variety of problems in drilling fluids, hydraulic fracturing fluids, water flooding, pipelines, and storage tanks. The primary microorganisms of concern...

... the cause. To solve the obstruction problem the system was treated downhole using acid and biocide. During this treatment dangerous amounts of H₂S were released. A study was initiated to understand the origin of the H₂S and to give options for microbiological treatment and growth...

... presents many difficulties. Oilfield waters vary greatly in chemical composition and biological activity. The salinity of produced waters can vary from 0.1% to 35% and can contain a variety of production chemicals including corrosion inhibitors, scale inhibitors, methanol and biocides. To illustrate...

... to reducing MIC-related damage and failure of oil and gas assets is to deploy pigging devices that physically remove biomass and scale from the inside walls of oilfield pipelines. In a process referred to as “chemical pigging,” the pigging device is accompanied by a dose of biocidal product meant to kill any...

... mitigate these damage mechanisms. Consequently, the methodology developed involved initially flushing the facilities with less saline water to remove deposits and sediments, and subsequently treating the entire facility with a mixture of corrosion inhibitor, oxygen scavenger and biocide in brackish water...

... decreasing the impact of H₂S production and on MIC as a whole. sulfate-reducing bacteria microbiologically influenced corrosion MIC hydrogen sulfide H₂S reservoir souring biocide INTRODUCTION Microbial reduction of sulfur-containing compounds results in the production of sulfide...

... Study A sessile regrowth study was performed to monitor the regrowth kinetics of a representative biofilm grown in fluids collected from the field line. 3,750ppm (µL/L) (1250ppm (µL/L) of active glutaraldehyde and 2500ppm (µL/L) of active ADBAC) of total active biocide was directly dosed...

... inhibitors, coatings and cathodic protection (CP). The localized corrosion rate model is affected by biocides, periodic cleaning of piping and vessel internal surfaces (PC), coatings and CP. This CP and coatings, both these corrosion control strategies, could help in reducing both, the uniform corrosion rate...

... of H₂S. Current chemical treatment options for remediation of biotic reservoir souring include targeting microbial populations with a biocide or using calcium nitrate to selectively grow microorganisms that metabolize nitrate instead of sulfur compounds. We have recently identified a number of novel...

...The following biocides were used in this study: Bellacide^® 300 ^† , which contains 5% of a phosphonium polyammonium blend (PPAB); Aqucar™ DB 20 ^† , which contains 20% 2,2-dibromo-3-nitrilopropionamide (DBNPA); Ucarcide^® 50 Antimicrobial...

... a significant amount of attention in regards to asset integrity, as well as U.S. government compliance. This is a comprehensive field case study of the effectiveness of various biocide applications and chemistries in conjunction with CO₂ acid gas corrosion inhibition and routine maintenance pigging...

...The SG hybrid material, embedded with biocides of interest, was freshly coated on mild steel panels to assess their anticorrosion and antifouling behavior in saline media. The surfaces of all biocide-embedded coated samples after curing appear homogenous and intact with no signs of immediate...

...Abstract Microbiologically influenced corrosion (MIC) is caused by problematic biofilms in many industries, especially the oil and gas industry. In this work, a novel peptide (labeled as “Peptide A”) was used to enhance tetrakis hydroxymethyl phosphonium sulfate (THPS) biocide to treat...