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1-20 of 61
Keywords: bacteria
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Proceedings Papers
Lessons Learned from New Stainless Steel Fabrication
Available to Purchase
... installation of a potable water system in a Gulf Coast petrochemical facility. Lessons will cover materials specifications, post fabrication cleanup, and prevention of MIC. Microbiologically Influenced Corrosion Pitting Stainless Steel Heat Tint Bacteria Biofilm Failure Analysis SRB INTRODUCTION...
Proceedings Papers
Microbiologically Influenced Corrosion by General Aerobic and Anaerobic Bacteria in Oil & Gas Separators
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... direction. Furthermore, investigation carried out by the Most Probable Number (MPN) Serial Dilution technique in accordance with NACE documents TM0194-2014 14 and TM0106-2016-SG 15 showed low to nil SRB counts, and very high General Aerobic Bacteria (GAB...
Proceedings Papers
On Site qPCR Application for Rapid Microbial Detection and Quantification
Available to Purchase
... of the shortfalls with current microbial enumeration technology and demonstrates that qPCR can provide an appropriate balance of speed and accuracy over other field-ready enumeration techniques for measurement of total bacteria or sulfate-reducing bacteria (SRB). The study also demonstrates the need to undertake...
Proceedings Papers
Relative Performance of Various 16S iTag Amplicon Sequencing Primer Pairs in Profiling Microbial Communities Relevant to Oil & Gas Operations
Available to PurchaseKathleen Manna, Joseph Moore, Kenneth Wunch, Ella Massie-Schuh, Rebecca A. Daly, Kelly Wrighton, Michael Wilkins
... (genus) (3%) Thermoanaerobacter (genus) (1%) Blvii28 wastewater-sludge grp (1%) Petrotoga (genus) (0.6%) Data not available Abstract The oil and gas industry's efforts to characterize microbial communities in oilfield process fluids has shed increasing light on the influence of bacteria and archaea...
Proceedings Papers
Biofilms in Industrial Water Systems: Metagenomic Insight on Biofilm Populations from a New Real-Time Biomonitoring System
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... chemical treatments, shear stress, and predators. Within the biofilm matrix, microorganisms thrive in conditions responsible for promoting a corrosive environment. The types of bacteria responsible for corrosion vary from system to system. In this study we used a novel biomonitoring system installed in 26...
Proceedings Papers
Development of Autonomous Microbe Sensor (AMS) at Seawater Injection System (SWIS)
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... time. To overcome the MIC challenges, Saudi Aramco, in collaboration with international company, has developed an Autonomous Microbe Sensor (AMS) technology (Bacteria Sensor) to detect & measure in real-time & on-line the bacteria counts in sea water injection system. AMS that is based...
Proceedings Papers
Endospore Production in Response to Microbiocides in the Microbiologically Influenced Corrosion Implicated Genus Clostridium
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...Abstract Based upon the observation that an oilfield appeared to possess an endospore-forming microbial population that was selected for by its microbiocide program, a hypothesis was formed that exposing endospore-capable bacteria to low dosing of microbiocides, particularly to those chemistries...
Proceedings Papers
Development and Field Application of a New Bacteria Monitoring Technique
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...Testing also determined that the method was not affected by the presence of hydrocarbons or production chemicals. Given that bacteria samples degrade and change shortly after collection the new method also required to be compatible with oil and gas field conditions. The new method...
Proceedings Papers
MIC Considerations for Cathodically Protected Encased Ductile Iron Pipelines: a Case Study
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... for microbiological analysis of samples collected from underneath the polyethylene encasement. The kit enumerates viable bacteria belonging to the following groups: anaerobic bacteria (ANA), acid-producing bacteria (APB), iron-related bacteria (IRB), low nutrient bacteria (LNB) – Slime formers and sulfate-reducing...
Proceedings Papers
A Case Study for MIC Evaluation and Mitigation in Two Argentinian Oilfields
Available to PurchaseEmerentiana Sianawati, Geert van der Kraan, Brandon E. L. Morris, Matheus Paschoalino, Juan M. Dominguez, Nelson Cardena, Pablo Bonifacio, Santiago Almada, Fernanda Ulloa
...Specific microorganisms, Sulfate Reducing Bacteria (SRB) and Acid Producing Bacteria (APB), isolated from the water samples were enriched applying commercial “bug bottles” and used as the test inoculum for a biocide selection study. DNA was extracted on-site from several locations and later...
Proceedings Papers
Identification of Compounds That Effectively Block Microbial H2S Production
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... of bacteria and archaea are capable of generating H2S from an array of sulfur-containing compounds, although the key enzymes involved are relatively well conserved. We have identified a class of inhibitory molecules that abrogate sulfidogenesis by numerous diverse microbial populations found within...
Proceedings Papers
Interpreting Omic Data for Microbially Influenced Corrosion: Lessons from a Case Study Involving a Seawater Injection System
Available to PurchaseCraig Bartling, Ph.D., Angela Minard-Smith, MS, Kate H. Kucharzyk, Ph.D., Jennifer Busch-Harris, Larry Mullins
... (base calling, quality calibration, cluster filtering, and demultiplexing) was performed on-instrument. metagenomics proteomics microbially influenced corrosion sequencing bacteria INTRODUCTION Microbiologically influenced corrosion (MIC) is a significant source of pitting corrosion affecting oil...
Proceedings Papers
Sustainable Biocide Formulations to Deliver Controlled Post Fracture Souring Management
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...THPS and 1,3-Dimethylol-5,5-dimethylhydantoin (DMDM Hydantoin) are both biocides often used individually, and very effectively, in many industrial applications to control the proliferation of undesired bacteria. THPS is already widely used in hydraulic fracturing, and provides a quick kill...
Proceedings Papers
Sea Water Cooler Tubes Corrosion and Leaks Due to Microbiologically Induced Corrosion
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...Abstract Refining industry cooling systems are prone to many different corrosion deterioration mechanisms, one of which is Microbiologically Influenced Corrosion (MIC). Bacteria are ubiquitous, they have been implicated in a number of major failures and their control measures have a high economic...
Proceedings Papers
Marine Crevice Corrosion of Stainless Steel Alloys under Biofilmed and Sterile Conditions
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... as to prevent bacterial activity. EXPERIMENTAL PROCEDURE Materials Attempting to set operational limits for stainless steels in terms of resistance to MIC is even more complex. Bacterial action plays a part in pitting and crevice corrosion in natural seawater; aerobic bacteria colonize the steel surface...
Proceedings Papers
The Selection and Performance of Oil and Gas Biocides for Extended Microbial Control
Available to PurchaseTerry M. Williams, Ph. D., Arvind Murali Mohan, Ph. D., Emmanuel Appiah Amponsah, Ph. D., Joseph Moore, Ph.D., Christine Schultz, Ella Massie-Schuh, Diamond Dyer, Phuc Pham, Philip Maun
... sandstone to 10 mL of the injection fluid. The injection fluid/biocide was incubated with the crushed sandstone for 24 hours (30°C) to allow ample time for adsorption to occur. Abstract It is well understood that sulfate reducing bacteria (SRB), acid-producing bacteria (APB), and facultative anaerobic...
Proceedings Papers
A Case Study Evaluating a Co-Injection Biocide Treatment of Hydraulic Fracturing Fluids Utilized in Oil and Gas Production
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... of treatments applied at 1:1 to 1:4 GLUT to DMO active ratios were highly synergistic against bacteria. This ratio provided a rapid kill of sulfate-reducing bacteria (SRB) and acid-producing bacteria (APB), as well as extended control in downhole conditions. Testing conditions were designed to simulate downhole...
Proceedings Papers
Don’t Just Blame the SRBs and APBs for MIC
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... (if not hundreds) of different genera of bacteria and archaea. Importantly, microbes can possess multiple types of metabolic pathways that can be utilized in different environments, therefore necessitating their inclusion in multiple classes: NACE 31205 provides detailed descriptions of many of these eight classes...
Proceedings Papers
Determining Effective Antimicrobial Treatments for Long-Term Protection of Hydrocarbon Reservoirs
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...-producing and sulfate-reducing bacteria) to evaluate extended biocide performance in water chemistries and temperatures that mimicked downhole environments. This paper discusses a summary of effective biocide treatments for the holistic protection of hydraulic fracturing operations from microbial...
Proceedings Papers
Data Mining to Prevent Microbiologically Influenced Corrosion?
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... on identifying the particular types of bacteria and archaea responsible for causing these issues. This knowledge will assist in the design of better prevention, treatment, and control strategies for microbial activity. A series of in-depth analyses were carried out on a database of field samples submitted...
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