... Biocide Acrolein Horizontal Well Microbial APB SRB ATP 16S rRNA Iron sulfide INTRODUCTION Testing was conducted based upon EPA and API standard test methods. ^{18 , 19} Iron and manganese counts are determined as part of a standard water analysis conducted on an inductively coupled...

... activity and spreading inside the HCB, consequently minimizing risks of biodeterioration and MIC inside HCB, even though environmental conditions outside of HCB were favorable for bacterial growth. MaCoTe Material Corrosion Test DGR aerobic bacteria anaerobic bacteria sulfate-reducing bacteria SRB...

... installation of a potable water system in a Gulf Coast petrochemical facility. Lessons will cover materials specifications, post fabrication cleanup, and prevention of MIC. Microbiologically Influenced Corrosion Pitting Stainless Steel Heat Tint Bacteria Biofilm Failure Analysis SRB INTRODUCTION...

... direction. Furthermore, investigation carried out by the Most Probable Number (MPN) Serial Dilution technique in accordance with NACE documents TM0194-2014 ¹⁴ and TM0106-2016-SG ¹⁵ showed low to nil SRB counts, and very high General Aerobic Bacteria (GAB...

...Zinc can also be corroded by proton when the zinc surface’s passive film is damaged by SRB. At pH 7, 2H⁺/H₂ potential (E^o' = −414 mV) is not much higher than that for Fe²⁺/Fe (E^o' = −447 mV), making proton attack of carbon...

...Research detailed in reference 6 described the influence of pulse increments of SRB (isolated from field sources) and Modified Postgate B (MPB) nutrient broth in laboratory test cells —in naturally aerated and partially de-aerated deionized water medium— on SRB proliferation and steel corrosion...

... begin conducting MMM-based analyses. DNA extraction quantitative PCR qPCR microbiologically influenced corrosion MIC sulfate-reducing prokaryote SRB iron-reducing bacteria IRB methanogen next-generation sequencing NGS 16 rRNA metagenomic analysis INTRODUCTION Optimization of qPCR Assays...

...Culture Methods Absrract Certain classes of microorganisms (e.g., sulfate reducing bacteria or SRB) have been linked to an increased risk of microbiologically influenced corrosion (MIC) and reservoir souring. The ability to quickly and accurately detect and enumerate MIC-associated microbes...

... or becomes diluted to more tolerable concentrations. This scenario, over time, would select for this population of bacteria to become the majority and render the microbiocide program ineffective. MIC SRB APB endospore microbiocide Clostridium bacteria Schaeffer-Fulton method INTRODUCTION...

...The civil infrastructure exposed in submerged environments can have high sulfate concentration that promote metabolic activities of SRB. Although MIC has not traditionally been a major durability concern for Florida’s coastal and inland bridges, recent findings from the Florida Department...

...INTRODUCTION Table 1 Summary of the microorganisms and growth media used for study Organism ATCC Number Metabolism Growth media Growth Condition Desulfovibrio desulfuricans subsp Desulfuricans ATCC 29577 SRB ATCC 1249 30° C anaerobic Desulfovibrio vulgaris subsp...

..., samples collected from failed pipe surfaces and samples from salt water disposal facilities, were collected in a comprehensive survey. Microbial activity was measured in parallel using four different bacterial quantification methods: 1) traditional MPN culture-based assay for SRB, APB, GHB (aka "bug...

... mL^-1 with considerable diversity. MIC of stainless steels AISI 304 and AISI 316 in natural anoxic groundwater containing enrichment of sulfate reducing bacteria (SRB) was studied over a one-year experimental period. Electrochemical techniques enabling real-time survey of corrosion...

... the findings demonstrating this bacteria type-damage pattern relationship. Table 3 Corrosion and Bacteria Data (High GAB) Fluid Coupon Retrieval Date Days Exposed Corrosion Rate (mpy) Sessile Bacteria (Counts/cm²) Visual Observation General Localized SRB GAB GAnB RCW 12/Mar...

...Laboratory testing for MIC is complicated by conflicting requirements. In totally anaerobic conditions there is essentially no cathodic process available to sustain corrosion of stainless steels; therefore, no corrosion is expected with or without active SRB. In fully aerated conditions, SRB...

...Abstract Microbial induced corrosion (MIC) is a phenomenon where by microorganisms presence and activities result in the degradation of a material or component. In particular for the oil and gas industry, various bacteria such as sulfate reducing bacteria (SRB), acid producing bacteria, general...

... for sulfate-reducing bacteria (SRB) and acid-producing bacteria (APB), rapid bacterial cell enumeration based on an adenosine triphosphate (ATP) quantification assay, epifluorescence microscopy, and molecular analysis of the microbial populations by DNA isolation and sequencing of 16S amplicons were directly...

.... Microbiological analyzes by inoculation (APB and SRB inoculation) and counted colonies per ml (UCF) were conducted in the field (in situ). The liquid sample selected to perform the laboratory test was taken from a positive vial for APB and SRB well. The liquid sample with positive APB and SRB activity was tested...

.... In this study, oilfield samples of predominantly Sulfate Reducing Bacteria (SRB), Acid Producing Bacteria (APB), and General Heterotrophic Bacteria (GHB) were subjected to four storage conditions: refrigeration (4°C), room temperature (25°C), 35°C, and daily cycling between 25°C and 35°C. The microbial...

... to demonstrate that growth of mesophilic and thermophilic sulfate-reducing prokaryotes, including sulfate-reducing bacteria (SRB) and sulfate-reducing archaea (SRA), could be supported. Experimental trials investigated the feasibility of monitoring the onset of reservoir souring by measuring microbially...