Microbially Influenced Corrosion is primarily caused by sessile microbes found within a biofilm. The ultimate goal of many biocide treatments is the removal of any biofilm within that system. These cells may be killed but remain in place, be removed but not killed, or may be both killed and removed. Many of the factors that determine the fate of sessile cells are poorly understood. For example, deciding to treat a system with a low dose of biocide for a long period of time or to treat with a high dose of biocide for a short period of time may yield drastically different results in terms of reducing the risk of microbially influenced corrosion. In this study, we show a comprehensive approach to growing in vitro biofilms to conduct sessile kill studies for product qualification.

Subject
Water, Viability, Reactors, Surfactants, Biofilms, Bacteria, Produced water, Corrosion coupons, Biocides, Microorganisms, Microbiologically influenced corrosion, Samples, Coupons
Keywords:
Biofilms, Sessile Kill Study, CDC Biofilm Reactor, CLSM, ATP quantification, Microbially Influenced Corrosion, MIC, Biofouling, Next-Generation DNA sequencing, Failure Analysis
© 2016 Association for Materials Protection and Performance (AMPP). All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means (electronic, mechanical, photocopying, recording, or otherwise) without the prior written permission of AMPP. Positions and opinions advanced in this work are those of the author(s) and not necessarily those of AMPP. Responsibility for the content of the work lies solely with the author(s).
2016
Association for Materials Protection and Performance (AMPP)
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