Pipeline steel coupons were exposed in a model system containing Enterobacter aerogenes, Clostridium acetobutylicum and Desulfovibrio desulfuricans. After various exposure periods, the coupons were preserved, embedded and examined using phase contrast and UV fluorescence microscopy, transmission electron microscopy and scanning electron microscopy. Corrosion pit initiation and growth were compared with biofilm characteristics, particularly with respect to the sulfate reducing bacteria (SRB). Distinct changes in SRB morphology were observed over time when the bacteria were involved in the corrosion. These morphological changes could significantly impact the manner in which SRB contribute to localized corrosion. Similar changes and resultant effects on corrosion were also documented in field samples from natural gas pipelines.

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