The biocidal efficacy of brominated hydantoins versus planktonic and biofilm bacteria was determined. Brominated hydantoins were effective at low concentrations versus planktonic bacteria and this activity was not affected by ammonia. Higher concentrations were required for efficacy versus biofilm bacteria. The efficacy of brominated hydantoins versus biofilm bacteria increased in the presence of ammonia. The biocidal and biofilm removal activity of bromo-chloro-dimethyl-hydantoin (BCDMH) was superior to that of bromo-chloro-ethylmethyl-hydantoin (BCEMH).

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