The biocidal action of ozone either on aerobic bacterial biofilms of Pseudomonas fluorescens or anaerobic biofilms of two strains of sulphate-reducing bacteria (Desulfovibrio vulgaris and Desulfovibrio desulfuricans) was studied in laboratory experiments within the concentration range of 0.2 to 0.5 ppm under quiescent conditions. Biofilms were formed on SAE 1020 carbon steel and on AISI type 304L stainless steel samples. The influence of the metal surface nature and the contact time on ozone biocidal effectivity as well as ozone effects on the electrochemical behavior of each metal were analyzed. In the case of stainless steel, ozone was effective not only to kill bacteria but also to facilitate the detachment of bacterial biofilms.

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