Laboratory experiments under well defined conditions (water chemistry, microbial strains, substrata composition, and experimental techniques) were made to assess the biocidal action of ozone on pure and mixed bacterial biofilms. Bacterial biofilms of Pseudomonas fluorescens, or anaerobic biofilms of two different strains of sulphate-reducing bacteria, were exposed to ozone concentrations within the range of 0.2 to 1 ppm under quiescent conditions. Biofilms were formed on SAE 1020 carbon steel and on AISI type 304L stainless steel samples. The effect of ozone on the corrosion behavior of these metals, as well as the possible synergistic effect of ozone/bromide mixtures were also studied. Ozone was effective not only to kill bacteria but also to facilitate the detachment of bacterial biofilms formed on stainless steel. However, this detachment was not observed on carbon steel samples. Preliminary experiments showed that ozone-bromide ion mixtures do not modify either the biocidal action or the corrosion behavior of the metals with respect to that obtained with ozone alone.

Subject
Scanning electron microscopy, Ions, Ozone, Mixtures, Biofilms, Incubation, Sodium chloride solution, Solutions, Oxygen, Carbon steel, Cooling water, Stainless steel, Corrosion potential
Keywords:
ozone, biofilms, sulphate-reducing bacteria, biocide, bromide, stainless steel, carbon steel
© 1994 Association for Materials Protection and Performance (AMPP). All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means (electronic, mechanical, photocopying, recording, or otherwise) without the prior written permission of AMPP. Positions and opinions advanced in this work are those of the author(s) and not necessarily those of AMPP. Responsibility for the content of the work lies solely with the author(s).
1994
Association for Materials Protection and Performance (AMPP)
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