Biofilm formation can create corrosion problems in industrial water systems. Control of biofilms is achieved most effectively when the mechanism of formation is understood. The use of traditional microbiological analyses such as plate counts and dipslides to analyze deposits provides insufficient information about viable cell content and their role in deposit formation. The ATP assay, a newer technology is more useful but only measures total living biomass. In order to assess the potential for biofouling in cooling water systems, novel staining and monitoring techniques have been developed. Staining technology allows characterization and assessment of biofilm composition. This staining methodology is used to complement ATP analysis of field samples. Case histories are used to illustrate the benefits of this approach.

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