DNA was extracted from a water sample taken from an offshore seawater injection system. DNA was also extracted from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the Polymerase Chain Reaction (PCR) and bacterial diversity was studied using Denaturing Gel Gradient Electrophoresis (DGGE). The obtained results showed that microbial evaluation was biased by the use of artificial culture media although recommended media were used, indicating that microbiological analysis of waters in industrial systems by culturing methods may not be adequate. Four facultative anaerobic bacteria were then isolated from the original sample: Pseudomonas sp., Aeromonas sp., Shewanella algae strains 1 and Shewanella algae 2. Linear Polarization Resistance evaluation of the corrosive activity of these bacteria indicated that they were more aggressive mixed in a consortium than in pure cultures. These bacteria are also suspected to resist the biocide treatment used in the studied seawater injection system.

Subject
Water, Materials, Polymerase chain reaction, Heavy water, Anaerobic bacteria, Sodium chloride, Seawater injection, Corrosion detection, Extracts, Sodium, Bacteria, Linear polarization resistance, Samples
Keywords:
Offshore seawater injection system, biocorrosion, facultative anaerobic bacteria, DGGE
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2005
Association for Materials Protection and Performance (AMPP)
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