A newly developed environmental scanning electron microscope (ESEM) coupled with an energy dispersive x-ray spectrometer (EDS) was used to characterize the topography and chemical composition of wet biofilms and corrosion products on metal surfaces in addition to spatial relationships between microorganisms, substratum and corrosion layers. Case studies are presented to demonstrate the applicability and advantages of ESEM/EDS technology in the investigation of microbiologically influenced corrosion (MIC) as compared to traditional methods.

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