Enzymes are produced by microorganisms to utilize the energy available in nutrients. The production of hydrogenase enzyme by the sulfate-reducing bacteria and other corrosive bacteria appears to be involved in MIC. A new test for hydrogenase enzyme has been developed and appears to detect the corrosion activity of a wide range of microorganisms. Further studies have indicated that the presence of cell-free extracts of bacteria enzymes can increase corrosion in mild steel.

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