A sulfide oxidase enzyme electrode is proposed for monitoring of biogenic sulfide resulting from the activities of sulfate-reducing bacteria (SRB). The enzyme which is immobilized in a carbon (graphite) paste with 1,1’ dimethylferrocene, catalyses the oxidation of sulfide to sulfur. Amperometric measurements were carried out at a fixed potential of 0.3 V, and room temperature, using Tris-HCl buffer (pH 7.5) as the electrolyte, and sodium sulfide solution as the substrate. Under deaerated conditions, the biosensor responded linearly to the tested sulfide concentration in the range of 0–130 ppm. The results indicate that the procedures adopted for the enzyme production and electrode development were reproducible.

Subject
Materials, Electrochemical cells, Probes, Graphite, Solutions, Pastes, Sulfide concentration, Bacteria, Carbon, Sulfides, Electrodes, Microbiologically influenced corrosion, Electrode potential
Keywords:
monitoring, sulfide, biosensor, sulfate-reducing bacteria
Government work published by the Association for Materials Protection and Performance (AMPP) with permission of the author(s). Positions and opinions advanced in this work are those of the author(s) and not necessarily those of AMPP. Responsibility for the content of the work lies solely with the author(s).
2008
GOV
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