Microbially-influenced corrosion (MIC) is a costly problem across several industries. The steadily rising use of advanced molecular biological methods to investigate MIC allowed ever deeper insights into the underlying microbial community structure and function. However, currently available technologies do not allow accurate detection and diagnosis of MIC in the field. The recent discovery of a special hydrogenase in certain corrosive methanogenic Archaea allowed for the development of a first-ever MIC biomarker termed here micH. The micH gene encodes the large subunit of a special [NiFe] hydrogenase involved in MIC. Here we describe the development of a recombinant antibody that enables the specific and sensitive detection of the MicH protein in Western blot immunoassays. Using a recombinant MicH protein we determined the lower limit of detection per assay to be around 0.3 fg MicH. The immunoassay was able to detect a strong signal for the MicH protein in micH-positive pure cultures of Methanobacterium-like. strain IM1 that was cultivated on iron granules, and the signal was over 500 times lower in a micH-negative Methanococcus maripaludis S2 culture. To further evaluate the ability to differentiate corrosive from noncorrosive microbial communities, we tested ten oil field enrichment cultures that showed a wide range of corrosion rates (0.02 mm/y to 0.48 mm/y). We detected the MicH protein in planktonic (36.5 pg/mL to 1473.5 pg/mL) and carbon steel biofilm samples (41.0 pg/cm2 to 7971.3 pg/cm2) from corrosive methanogenic enrichments (0.17 mm/y to 0.48 mm/y) but did not detect MicH in any of the noncorrosive tests (<0.08 mm/y) despite methanogenic activity. The results indicate that corrosion was likely caused by methanogenic Archaea expressing a corrosive [NiFe] hydrogenase and that the newly developed MicH-specific immunoassay can detect and monitor their activity. The development of specific and sensitive immunoassays to detect a MIC biomarker allows corrosion scientists and field practitioners to detect and monitor the activity of corrosive methanogenic Archaea.
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1 January 2025
Research Article|
December 13 2024
Development of an Immunoassay for the Detection and Diagnosis of Microbially Influenced Corrosion Caused by Methanogenic Archaea
Sven Lahme
;
Sven Lahme
‡
*ExxonMobil Technology and Engineering Company, 22777 Springwoods Village Parkway, Spring, Texas, 77389.
‡Corresponding author. E-mail: [email protected].
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Jorge Mantilla Aguas
Jorge Mantilla Aguas
*ExxonMobil Technology and Engineering Company, 22777 Springwoods Village Parkway, Spring, Texas, 77389.
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‡Corresponding author. E-mail: [email protected].
Online ISSN: 1938-159X
Print ISSN: 0010-9312
© 2025, AMPP
2025
CORROSION (2025) 81 (1): 21–26.
Citation
Sven Lahme, Jorge Mantilla Aguas; Development of an Immunoassay for the Detection and Diagnosis of Microbially Influenced Corrosion Caused by Methanogenic Archaea. CORROSION 1 January 2025; 81 (1): 21–26. https://doi.org/10.5006/4673
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